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DOT Code: 022.381-010

Industry: beverage

Selects and cultivates yeast cells to develop pure yeast culture for brewing beer and malt liquors: Samples beer in fermenting stages to select specimens containing yeast cells having specific reproduction characteristics. Prepares cover glasses and slides with smears of yeast preparation taken from beer samples. Examines slides under microscope to isolate specific cells and record position of cells. Incubates slides to allow colonies of cells to form. Inoculates cells with hopped wort, using sterile needle to transfer cells from slides to flasks containing wort. Cultivates cells in flasks by incubation and propagates single cell cultures by transferring cells to additional flasks. Siphons developing yeast culture from flasks to tanks and pumps yeast culture from tanks to sterile barrels. Seals barrels with sterile bung and packs sealed barrels in dry ice to prevent yeast degeneration. Cleans and sterilizes equipment.

GOE: 02.04.02 STRENGTH: M GED: R4 M2 L4 SVP: 5 DLU: 77